is the Rubenstein western blot validated?
” The following is a summary of the current situation relative to a finding of an atypical TSE in sheep in Vermont. On 10 Jul 2000, 4 sheep from Vermont flocks tested positive for a transmissible spongiform encephalopathy (TSE). These 4 positive results, plus 2 suspicious results, were obtained on a Western blot analysis. The samples were processed as described in the following publication: Rubenstein, et al., Arch. Virol. (1994) 139:301-311. The antibody used for the analysis was the polyclonal antisera derived from the rabbit anti-C57BL mouse PrP antibody, referred to as rabbit anti-CMPrP8 in the following publication: Kascsak, et al., Brown, F. editor, Transmissible Spongiform Encephalopathies – Impact on Animal and Human Health, Dev. Biol. Stand. Basel, Karger (1993) 80:141-151. This is a validated recognized test, performed at a USDA cooperating laboratory….” Lisa A Ferguson Sr. Staff Veterinarian USDA, APHIS, Veterinary Services Riverdale, MD Joseph F. Annelli, DVM, MS Chief St
