Is the quantitation of the cDNA amplification product included in the automated methods?
The automated methods include the preparation of a plate containing an aliquot of amplified product for quantitation but the actual measurement and subsequent importation of the data requires manual intervention. The methods were written with the view that a minimal approach will be of greater utility for customers with constraints on instrument configuration. Adaptation to more complex configurations can be accomplished but it is best done on a case by case basis. The documentation package also includes helpful worksheets and instructions for cDNA quantitation.
