Is the orientation of the western blot within the blot holder important for immunodetection with the SNAP i.d. System?
• Proper positioning of the membrane is critical for optimal performance. The protein side of the western blot (i.e., the side of the membrane that contacted the polyacrylamide gel) should always be in direct contact with the blot holder membrane (see diagrams in the User Guide). Blots placed with protein side away from the blot holder membrane will show significantly lower signal.
• Proper positioning of the membrane is critical for optimal performance. The protein side of the western blot (i.e., the side of the membrane that contacted the polyacrylamide gel) should always be in direct contact with the blot holder membrane (see diagrams in the User Guide). Blots placed with protein side away from the blot holder membrane will show significantly lower signal. • The blot spacer is required to keep the blot from contacting the grid of the blot holder. It ensures the even distribution of the reagents to the blot.
