Is the N-terminus the only place where a signal sequence leads to export of the protein – is it possible that a protein fused to MBP could contain a fortuitous signal sequence?
It probably would be possible to have some internal region that acts as a signal sequence. It’s also possible that hydrophobic regions in the protein lead to association with the membrane, which in turn leads to limited cell lysis during preparation of the periplasmic fraction. Neither one is very common, but perhaps the latter is seen more often.
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