Is the miRCURY LNA™ real-time PCR system compatible with other SYBR Green® master mixes?
We do not recommend using other master mixes for real-time PCR analysis with the miRCURY LNA™ primer sets. The primer sets have been optimized and validated using the miRCURY LNA™ microRNA SYBR Green® Master mix and the performance of the primer sets could be compromised by using a different master mix (which may contain different salt and/or enzyme concentrations).