Is the MeDIP kit suitable for comparing DNA methylation of bacterial DNA?
Our MeDIP kit can be used for the DNA methylation studies in different organisms. In the kit we use the antibody raised against 5-methyl-cytosine. In bacteria the methylation happens on cytosine and adenosine residue; so our kit can be used only for cytosine methylation studies. If you decide to use this kit (so if the methylation concern the cytosine) you can just follow the protocol. Nevertheless I would like to add two informations: There are two controls included in the kit: positive and negative (methylated DNA and unmethylated DNA) and two primers pairs for these controls. You can definitively use these controls with the primers during the protocol. But there are two others primers included – Alpha X1 (or TSH2B) and GAPDH, which are human specific, so you should replace them by the primers specific for bacterial region of interest.
