Is the list of plant-specific proteins exhaustive?
NO. This list of putative plant-specific proteins is not exhaustive for several reasons: • We used rather conservative criterias to define the putative plant-specific proteins. Therefore it is possible that true plant-specific proteins were discarded at some of the steps of our analysis pipeline. • ESTs comprise the largest pool of sequence data for many plant species and contain portions of transcripts from many uncharacterized genes. However EST sequences are fragments of genes, and EST populations often do not have good representation of genes expressed under special conditions or in few cell types. • We focused on protein-coding genes. However many genes encoding RNAs that are not translated and function as RNAs are suspected to be kingdom specific (e.g. Macintosh et al. Plant Physiol. 127, 765-776. 2002).
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