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Is the kit compatible with lysing enzymes if sonication does not work?

April 26, 2017compatible enzymes kit lysing sonication

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Is the kit compatible with lysing enzymes if sonication does not work?

1 Answer

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10 Posted

Yes, sheared chromatin can also be made by enzymatic (Micrococcal nuclease, MNase) digestion (reagents/protocol not provided in kit). The amount and duration of the MNase treatment will have to be optimized by the user depending on the cell line.