Is the IEF of protein samples labelled with CyDye™ DIGE Fluor minimal dyes more difficult or technically demanding?
Because the pI is unaffected, IEF separation is no more technically demanding than for unlabelled proteins. CyDye DIGE Fluor minimal dyes have the advantage of being highly sensitive, with a wider dynamic range than other techniques such as CoomassieTM Blue and silver staining. However, with all fluorescence imaging techniques, consideration is required to avoid the inclusion of components that either fluoresce or have the potential to quench fluorescence.
Related Questions
- What protein concentration range is recommended prior to the start of labelling with CyDye™ DIGE Fluor minimal dyes?
- Is the IEF of protein samples labelled with CyDye™ DIGE Fluor minimal dyes more difficult or technically demanding?
- How long will my CyDye™ DIGE Fluor minimal dyes keep once I have reconstituted them?
