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Is the hot-start enzyme contained in ABsolute Fast QPCR Master Mix as stringent as the enzyme contained in ABsolute Blue QPCR Master Mixes?

April 26, 2017BLUE enzyme fast hot-start master mix qPCR stringent

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Is the hot-start enzyme contained in ABsolute Fast QPCR Master Mix as stringent as the enzyme contained in ABsolute Blue QPCR Master Mixes?

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Yes, the ABsolute Fast contains a specially modified hot-start enzyme, Thermo-Fast, which prevents non-specific amplification during reaction set-up, while only requiring 5 minutes activation at 95°C.