Is the gel submerged under the buffer (i.e. is the gel in direct contact with the buffer)?
“The samples migrated but I don’t see any separation.” Was the gel stained and/or destained after electrophoresis? Stain with InstaStain® Methylene Blue for 15 minutes and destain with several rinses of warm (37°C) distilled water. For InstaStain® Ethidium Bromide, stain for 2-5 minutes and place the gel on a transilluminator (254 – 302 nm) to visualize the DNA.
