Is the FastPrep System designed for the isolation of enzymes? Is the enzymatic activity preserved?
The FastPrep system is designed for the isolation of enzymes from any sample. To preserve enzymatic activity, it is recommended to use Lysing Matrix tubes with large beads like ¼” ceramic beads included in Lysing Matrix A tubes and to homogenize samples for short times with incubation on ice for at least 2 minutes between successive FastPrep® homogenizations in order to prevent overheating of the sample. Bao J.R. et al. (Can.J.Plant Pathol. (2002), Vol 24: 340-348) used the FastPrep System for the homogenization of tomato roots and isolation of GUS enzyme and demonstrated that this method is yielding the highest enzymatic activity compared to other grinding methods: Abstract: Insertion of beta-D-glucuronidase (GUS) reporter gene has been found to be useful for detection, quantitation, and monitoring of plant-associated fungi in their environment. GUS was extracted from tomato roots inoculated with a nonpathogenic strain of Fusarium oxysporum, 70T01, that had been genetically modified t
