Is the FastPrep®-24 System suitable for lysis of spores?
Bacterial and fungal spores either in culture or in environmental samples are successfully lysed with the FastPrep®-24 system. Bacillus Subtilis spores in suspension in Lysing Matrix B tubes are processed 3 times for 40 seconds at speed 6.0 m/s with 1 minute cooling on ice between each run. 98% spore lysis was confirmed by microscopy. mHudson K.D. et al. (J. Bacteriol. (2005), Vol 183: 4317-4322) isolated proteins from Bacillus Subtilis spores for Western Blotting and Keijser B.J.F. et al. (J. Bacteriol (2007), Vol 189: 3624-3634) purified RNA from cultures of Bacillus Subtilis spores with the FastRNA® Pro Blue Kit and used total RNA for reverse transcription, labelling and hybridization on micro-array slides. Anthrax is one of the most dangerous zoonotic infectious disease and has been the first candidate for biological weaponry for over 80 years. It is very difficult to detect anthrax DNA from soil because of the presence of humic acid and many other nonsporulated and sporulated bact
