Is the FastPrep®-24 System designed for DNA extraction from Cryptosporidium oocysts?
There are three main problems associated with the isolation of DNA from Cryptosporidium organisms: (i) the extreme robustness of the oocysts (ii) the different physical and chemical nature of the matrices (faeces, water, food, soil) and their richness in PCR inhibitors (iii) the low number of oocysts usually present in environmental samples. The reduction or removal of PCR inhibitors is an essential component in the molecular detection of Cryptosporidium in faecal and environmental samples. Currently, pathogen isolation by Immuno Magnetic Separation (IMS) and culture enrichment prior to DNA extraction are standard procedures to eliminate or reduce PCR inhibitors. These methods, however, become impractical for organisms that have no IMS procedures or that cannot be cultured. The use of IMS is also expensive, and this limits the use of samples mostly to single organism detection. Thus, the development of methods for direct extraction of PCR quality DNA is important for the detection of p
