Is the collection of data from peripheral nervous tissues and/or neuroeffector organs technically feasible?
” There was some discussion of the difficulties in obtaining homogeneous, consistent tissue preparations (e.g., skeletal muscle, diaphragm) for measuring cholinesterase activity. Several members of the Panel did consider it technically feasible to routinely conduct cholinesterase assays in such tissues, however. This information would be extremely important in establishing the value of blood cholinesterase information in predicting peripheral effects of anticholinesterases or replacing that information, at least in animal tests. 9. What factors are important to the conduct of that testing” The most important factors identified by the Panel were a) standardized and reproducible dissection and homogenization of the tissue; b) use of assays that can be conducted with minimal tissue dilution (critical in dealing with carbamate inhibitors), c) selection of tissues representing the most toxicologically relevant targets; d) t me elapsing between collection and assay; e) standardization of tis
