Is the 0.1% Triton X-100 in the Column Buffer necessary or could it be replaced by a comparable concentration of other detergent?
0.1% Triton X-100 is not necessary in the column buffer. High salt concentrations (up to 2 M NaCl tested), Triton X-100 (up to 0.5%), Tween 20 (up to 0.2% tested), and 50 mM EDTA had little effect on chitin binding or intein cleavage. However comparable concentrations of other detergents have not been tested yet. We recommend that other detergents be tested using the MBP fusion protein which can be expressed from the pMYB5 control plasmid (NEB#N6906S, supplied in the IMPACT-CN kit) or pTWIN-MBP1(NEB#N6953S, supplied in the IMPACT-TWIN kit).
Related Questions
- Is the 0.1% Triton X-100 in the Column Buffer necessary or could it be replaced by a comparable concentration of another detergent?
- Is the 0.1% Triton X-100 in the Column Buffer necessary or could it be replaced by a comparable concentration of other detergent?
- Can I substitute a different buffer and/or salt concentration in the column buffer?
