Is TAG a functional codon stop in Dictyostelium genetic code?
-Francesco Dondero, University of Piemonte Orientale, Italy, 20 Jul 1999 • Francesco, We frequently use TGA stop codon for Dicty expression and have confirmed the protein’s authentic C terminus. TAA is of course the stop codon used by virtually all Dicty genes. We have never used TAG, but Christophe Reymond mentioned some problems with it when expressing the circumsporozoite antigen from Plasmodium. -Martin Slade, 21 Jul 1999 [TOP] [INDEX] Terminators: What is the shortest length of sequence that anyone has shown to work as a transcriptional terminator? Most of the ones I use are rather large and very AT-rich. -Robert Insall, The University of Birmingham, UK, 13 Dec 1999 • Hello Robert, Recently I faced similar question by myself. What I experienced was that, when I simply put a termination codon (TAA) at the end of sequence to be expressed, actually the protein (in this case A15-driven GFP fusion) was expressed fine without terminator. I am not sure if this situation is reproducible a
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- Is TAG a functional codon stop in Dictyostelium genetic code?
