Is SNAP i.d. compatible with blocking reagents other than NFDM?
Blocker CompatibleRecommended Concentration Non-fat/low fat dry milk yes, ≤ 0.5%0.5% Casein, N-Z-Amine AS (Sigma) yes, ≤ 5%1% Bovine Serum Albumin (BSA) yes, ≤ 5%1% PVP-40 (Polyvinylpyrrolidone)yes, ≤ 1%1% Immunoblot Blocking Reagentyes, ≤ 0.5%0.5% BLOT-QuickBlockerTM Reagentyes, ≤ 0.5%0.5%, pre-filter ChemiBLOCKERTM Reagent yes ≤ 50% SEA BLOCK Blocking Buffer (Pierce) yesundiluted SuperBlock® Blocking Buffer (Pierce) yesundiluted Li-Cor® Odyssey® Blocking Buffer yesundiluted Gelatinnot compatibleN/A Antibody addition Q. How much primary and secondary antibody should I add? • If you know the concentration used in standard immunodetection, use 3- to 5-fold higher concentration in 1/3 of the volume (3 mL for single well blot holder). Further optimization will likely be required. See application note entitled: “Triple Well Blot Holder as a Tool for 30-Minute Western Blotting Optimization with the SNAP i.d. Protein Detection System” (AN1965EN00). Q. What concentration of antibody should I
