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Is recombinant AAV replication deficient?

April 26, 2017AAV deficient recombinant replication

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Is recombinant AAV replication deficient?

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For wild type AAV, replication is at extremely low efficiency, without the presence of helper virus, such as adenovirus. For recombinant adeno-associated virus produced these days, the replication and capsid genes are provided in trans (in pRep/Cap plasmid), and only the 2 ITRs of AAV genome is left and packaged into virion, while the adenovirus genes required are provided either provided by adenovirus or another plasmid, the likelihood for a recombinant AAV to replicate is theoretically impossible. This is in similar scheme to lentiviral vectors produced these days.