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Is pLKO.1 vector a HIV-based vector and if so are there any biosafety issues?

April 26, 2017Biosafety plko.1 vector

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Is pLKO.1 vector a HIV-based vector and if so are there any biosafety issues?

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The pLKO.1 vector is a lentiviral (HIV)-based plasmid. The vector is regarded as a biosafety level 2 material and safe to use due to its modified features (deletion of a number of accessory genes implicated in the virulence of HIV, minimal genome of the viral particles, non-replicating and self-inactivation features), making it incapable of producing virus once infected into the host cell. Please consult with your institution’s biosafety officer on specific requirements.