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Is milk a good blocking reagent to use with SuperSignal West Pico Substrate?

April 26, 2017blocking good milk Pico Reagent Substrate supersignal West

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Is milk a good blocking reagent to use with SuperSignal West Pico Substrate?

1 Answer

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Yes and no. Milk contains variable amounts of biotin so it should not be used with avidin/biotin detection systems. Milk also contains varying amounts of phosphoproteins that may make interfere with anti-phosphotyrosine procedures. A variety of blocking buffers are compatible with the substrate.