Is microdialysis a popular method of crystallizing proteins?
A very large and vocal majority cried out, “no”! Attendees felt that dialysis is a good approach, but requires too much sample, and is just too difficult to set up using traditional methods. People would use the method if there was a better way to set up the experiments. A metal frame that holds the membrane flat and eases set up was described by one researcher. This frame eliminates the bubbles that can form during microdialysis experiments and block the liquid diffusion across the membrane. Another person described a method using Eppendorf microcentrifuge tubes to set up dialysis experiments using 10microL of sample.
