Is Melon Gel Purification a viable alternative to Protein A and Protein G?
Yes. 90% recovery of IgG and greater than 80% purity are typically observed for a single purification. Although IgA and IgM may be present with the purified IgG, a high percentage (> 80%) of IgA and IgM binds to the support. This high binding combined with the fact that IgA and IgM are present in low abundance in serum results in low levels of these components in the purified IgG.