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Is mechanical abrasion necessary with P-PER?

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Is mechanical abrasion necessary with P-PER?

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Yes, you still need mechanical abrasion to disrupt the cell wall of plant cells. The mesh bag in the kit works well for soft plant tissues (e.g., leaves, flowers). You can use a pestle along with the mesh bag to disrupt harder tissues; place the tissue in the bag with the P-PER reagent, then tap the outside of the bag with the pestle.

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