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Is it recommended to generate DCs from CD14+ cells or from CD34+ cells?

April 26, 2017cd14+ cd34+ cells DCs recommended

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Is it recommended to generate DCs from CD14+ cells or from CD34+ cells?

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Generally, both strategies can be used to generate DCs . However, the choice for one of the strategies should be made, depending on the planned experiment or application, since the obtained DCs differ in certain aspects. Monocyte-derived DCs can be loaded very efficiently with antigen. In contrast, CD34+ progenitor cell derived DCs are more suitable for retroviral transduction due to their proliferative activity.