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Is it possible to use primer of different length or annealing temperature?

April 26, 2017annealing different LENGTH possible primer temperature

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Is it possible to use primer of different length or annealing temperature?

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Posted

PRIMERFOX is kept designedly very simple. You just need to enter only few paramters, which makes it so simple and quick. Taking care of ranges of parameters as length or annealing temperature would complicate the using very much. So PRIMERFOX did not handle parameter ranges. But there is a way to combine primer of different PRIMERFOX queries. Just switch to the list view on the result page and copy the lists of forward and reverse primer to MS Excel (e.g.). Now you can easily calculate the PCR product length by the formula L = position(reverse primer) – position(forward primer) – length(forward primer) So you can easily handle different PRIMERFOX queries and you will find the primer combination you are looking for. If you wish to add more parameters for your primer calculation ask info@primerfox.com for implementation.