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Is it possible to use multiple enzymes in one map? Is each restriction enzyme used in an individual digest?

April 26, 2017digest enzyme enzymes individual map possible Used

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Is it possible to use multiple enzymes in one map? Is each restriction enzyme used in an individual digest?

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Maps are generated using one restriction enzyme per Optical Map. However some Customers find that it is advantageous to have Optical Maps generated using different restriction enzymes, particularly for sequence closure projects. If all sequence contigs are not placed with a particular enzyme, it is possible another enzyme may complete the contig placement.