Is it possible to use more than one antibody when performing ChIP or do you recommend using only one antibody per procedure?
Yes, many researchers combine multiple antibodies in their ChIP reaction to screen for antibodies that work well. If positive results are observed from this combined antibody approach, one can go back and perform ChIP using individual antibodies against the protein of interest. You can also perform experiments to examine the binding sites for a multi-protein complex by using a pooled sample of antibodies against all subunits of the complex.
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