Is it possible to use DNA extracted from paraffin coupes for MLPA?
Yes. You can download a protocol for DNA extraction from formaldehyde-treated, paraffin-embedded tissues (FFPE) here. We have been notified that the extraction of DNA from paraffin by other methods did not always result in satisfactory results. DNA extracted from FFPE tissue often contains contaminants. We therefore recommend comparing DNA test samples to DNA samples from healthy FFPE tissue. This way, it can be excluded that findings on a test DNA sample are in fact due the effects of the FFPE treatment. Formaldehyde cross-links should be destroyed by heat treatment; DNA should preferably be degraded to smaller fragments and be liberated from the tissue by protease treatment, before any tissue constituents are removed by centrifugation or phenol extraction.
