Is it possible to use different filters than 570 nm and 600 nm for measuring cell growth by absorbance?
In the general method section of the datasheet, the extinction coefficients are given for filters at 540 nm, 570 nm, 600 nm and 630 nm. Rather than deriving molecular extinction coefficients for other filters, which may be subject to error, a simple and accurate method has been devised, to calculate the amount of reduced alamarBlue® present, for any filter combination. (Personal communication of Dr. Geier (4) and in independently derived calculations by Goegan et. al. (1995) (5). For more information, please refer to Example calculations for using different filters in the Methods and Calculations section.
