Is it possible to sonicate DNA with the Bioruptor® and achieve the fragments between 2 kb and 5 kb?
Diagenode has not tested protocols to get DNA of 2-5 kb. However, we have conducted time course experiments on DNA shearing which have shown that after 1 minute at low power, we achieve an average DNA size of 800-1000 bp. Thus, it is possible to get larger fragments by reducing sonication time. We recommend your own optimization for such applications.
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