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Is it possible to run a multiplex RT-PCR on the ABI PRISM® 7000 or ABI Prism® 7900HT Sequence Detection System, and can I use more than two dyes in a single tube?

April 26, 2017Abi detection multiplex possible rt-pcr run sequence

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Is it possible to run a multiplex RT-PCR on the ABI PRISM® 7000 or ABI Prism® 7900HT Sequence Detection System, and can I use more than two dyes in a single tube?

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The ABI PRISM® 7000 or ABI Prism® 7900HT is capable of performing a multiplex RT-PCR assay. Currently, AB only supports assays using no more than two reporter dyes in a single assay. The analysis software has limited abilities to analyze two standard curves at one time, although two standard curves can be run on the same plate. To analyze two standard curves it is necessary to Export the data and re-plot the data in a spreadsheet program. To understand how to design a multiplex assay you should refer to User Bulletin #5: Multiplex PCR with TaqMan Vic Probes.