Is it possible to make soluble peptides with the AutoSpot?
Several people have worked on cleaving peptides from the SPOT membrane with different success. The major problem was always to get rid of the side chain protection groups while having the peptides still attached to the cellulose. Ronald Frank at GBF in Braunschweig, Germany, developed safety-catch linkers for this and he should have the best expertise. There is also a methods paper by Holger Wenschuh at Jerini Bio Tools, Berlin, which contains a number of protocols.
