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Is it possible to make a quantitative comparison of two samples in which the total protein concentration is different?

April 26, 2017comparison concentration different possible protein quantitative samples total

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Is it possible to make a quantitative comparison of two samples in which the total protein concentration is different?

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Ideally, experimental samples should be diluted to the same concentration prior to labelling. However, if this is not possible (e.g. the concentration of one of the samples is very low) a quantitative comparison can still be made providing all samples are labelled using the recommended ratio of 50 g protein per 400 pmol dye. The same amounts of sample labelled with each CyDye™ DIGE Fluor can then be combined for loading on the IPG strip for quantitative comparison.