Is it possible to isolate RNA from paraffin-embedded tissues with the FastPrep System?
Tissue samples to be used in microscopic and histological analyses are often preserved by embedding in paraffin. However, the presence of paraffin may interfere with isolation of RNA. For isolation of RNA from paraffin-embedded tissues, we recommend removing the paraffin by xylene extraction before proceeding with the FastRNA Pro procedure. Materials: – Xylene – Ethanol (100%, 95%, 70% solutions) – Distilled water – Glass jars or other solvent containers (such as Coplin staining dish) Procedure: 1. Section paraffin blocks at 5-10 microns 2. Place sections in a water bath at 42 °C to and eliminate any folds and wrinkles 3. Mount sections onto glass slides and let air dry overnight at room temperature. If sections do not adhere to the slides, they can be incubated at 42 °C for up to 8 hours. 4.
