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Is it possible to freeze the cells immediately after the collection and work with them later, after thawing?

April 26, 2017cells collection freeze immediately later possible Thawing

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Is it possible to freeze the cells immediately after the collection and work with them later, after thawing?

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The best is to work with fresh material, because we never know what influence on biological sample can have freezing/thawing. However, if you need to freeze your cells before performing your immunoprecipitation, we recommend that you compare the effects of freezing a sample to the results from a fresh sample. This means that you should perform the MeDIP with fresh and frozen samples in parallel and compare the results to account for any variation in. We do not recommend using frozen cells without comparing them with freshly collected cells!