Is it possible to extract DNA from feces with the FastPrep System?
Several studies report the isolation of inhibitor-free DNA from feces with the FastPrep® System for analysis of their bacterial content. Layton A. et al. (Appl.Environ.Microbiol. (2006), Vol 72: 4214-4224) and Ott S.J. et al. (J.Clin.Microbiol. (2004), Vol 42: 2566-2572) extracted DNA from human and animal feces with the FastDNA® SPIN Kit for Soil for real-time PCR assays and detection of bacterial species. On the other side, Tannock G.W. et al. (Appl.Environ.Microbiol. (2000), Vol 66: 2578-2588) and Requena T. et al. (Appl.Environ.Microbiol. (2002), Vol 68:2420-2427) extracted DNA from 1ml human fecal sample with the FastDNA® Kit to monitor the composition of the fecal microflora by PCRDGGE. Protocol for RNA extraction from murine corneas: Corneas are excised from frozen eyes of mice, and RNA is prepared using the FastRNA® Pro Green kit. Each cornea is placed in 0.8 ml RNApro™ m solution and homogenized with Lysing Matrix D in a FastPrep® instrument at setting 6.0 for 40 s. After cool
