Is it possible to create a MIDI file from polyphonic audio recordings?
Yes. With Melodyne editor it is indeed possible to detect single notes in a polyphonic recording and to export them as a MIDI file. This is not yet possible, however, in Melodyne studio or Melodyne assistant. Here, the source audio file must be monophonic. The playing technique is crucial, too: Sustained notes, coming from open strings on a guitar for example, have to be damped to maintain a monophonic signal. Melodyne essential RTAS doesn’t have the Audio-to-MIDI feature.