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Is it possible for me to design a synthetic probe for a specific gene and add it to an existing SALSA MLPA probemix?

April 26, 2017design existing gene mlpa possible probe salsa synthetic

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Is it possible for me to design a synthetic probe for a specific gene and add it to an existing SALSA MLPA probemix?

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Yes, we have successfully added completely synthetic probes generating amplification products with lengths of 112, 118 and 124 nt to our probemixes. Detailed guidelines on synthetic probe design are available here. Most SALSA MLPA probe mixes contain a synthetic IL1B specific probe that results in a 92 nt amplification product. Do not add more than 2 femtomoles of each probe oligonucleotide in each MLPA reaction. It is also possible to combine your own home-made synthetic probemix with our SALSA MLPA reference probemixes P200 or P300. These kits contain control fragments and longer reference probes.