Is it OK to move the transactivator in another vector?
The expression level of the transactivator is critical: you can put it in any vector, but make sure is under a strong promoter. • Q: Does the system work in vivo? A: The MLV LTRs used to drive expression of the transactivator is much less strong in vivo than in vitro. Furthermore its activity may vary with the type and the differentiation state of the cells. In summary, it is quite possible that even cells that display exquisite regulation in vitro will misbehave once transplanted in vivo.
