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Is it necessary to use feeder layer cells, coated plates or conditioned medium with CnT-media?

April 26, 2017cells Coated conditioned feeder medium necessary plates

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Is it necessary to use feeder layer cells, coated plates or conditioned medium with CnT-media?

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No, the CnT-media are designed to be used without feeder layer cells, plate coating or conditioned medium. For the growth of epithelial cells in CnT-medium it is not necessary to use any of the above mentioned techniques.