Is it necessary to extensively wash or boil the Slide-A-Lyzer Cassette before use to remove contaminants?
No. The Slide-A-Lyzer Cassette membrane requires no pretreatment. Trace metals are present in all Slide-A-Lyzer Cassette membranes. The 7,000 MWCO membranes contain 13% glycerol, 10,000 MWCO membranes contain 12% glycerol and 10,000 MWCO membranes contain 21% glycerol. These items will be lost during the dialysis process. To remove these items before introducing the sample, dialyze against water or a buffer for 10-15 minutes.
