Is it necessary to check RNA in Denaturing agarose gel systems?
It is preferable that you check your RNA on a denaturing gel. Denaturing agarose gel systems include formaldehyde and MOPs buffer to denature the RNA so that molecules will run by size. The 28S and 18S rRNA bands are visualized by ethidium bromide staining 5. Why is it necessary to purify RNA? Since contaminants like genomic DNA could hinder at a later steps in a microarray experiment it is necessary to purify RNA. In expression experiments the quality of the expression data depends on the purity and amount of RNA. Our facility has completed extensive research with various purification methods and provides services and suggestions to assist you with producing high quality RNA samples. 6. How should RNA be stored? RNA must be stored in -20C for short term storage and for long term -80C. 7. What are the time durations of these services? We assure you to give best results in minimum possible time although it entirely depends on the project /service requested. 8. How should I send RNA samp