Is it feasible to express cDNA from a lentiviral transfer vector normally used for shRNA expression?
Yes, it is feasible, but first the promoter within the transfer vector must be changed. Most shRNA expressing lentiviral vectors such as pLKO.1 use U6 promoters in order to drive RNA pol III transcription of shRNAs or sometimes the H1 promoter. cDNA expression requires the use of a RNA pol II promoter such as CMV or RSV.
