Is it always necessary to use a guard column with a TSK- GEL analytical column?
A guard column is generally recommended because it protects the analytical column from a major contamination of the HPLC, e.g. pump seal failure. It also protects against inadvertent overloading of the column because a guard column is both a particulate AND molecular filter. However, if the mobile phase and sample are filtered to 0.22- 0.45 micron a guard column may not be necessary. The user still, however, exposes the column to the potential contamination from the HPLC system.
