Is it also possible to use less than the specified cuvette filling volume for electroporation?
Yes. The specified cuvette volume (e.g. 100 µl for a 1 mm cuvette) is the maximum volume and also the optimum volume for the electroporation. Smaller samples can also be used, as long as the electrodes are connected through liquid. It is only necessary to be sure that the field strength during a pulse in the cuvette is less than 10,000 V/cm. Above this value, electric sparkover can occur through the air.
