Is doing a volume check prior to spotting my plates an important step in the overall Genotyping process?
• Yes! The chip is what bridges the gap between the reaction plate and the mass spectrometric analysis. If material is not properly spotted on the chip, you will have bad results. Make sure your liquid level is the same for all wells (no evaporation problem). Make sure your pins have been conditioned properly (daily and weekly maintenance). Check the resin in the well to make sure that it is all at the bottom of the well. You can find how to perform a volume check in your nanodispenser’s manual. The volume range for good results is 15-40 nL. An average volume of 25 nL is a good target to shoot for. If you observe high pin to pin variability during volume check, clean and condition your pins. Plate Editor in Typer 3.4 does not properly apply sample groups. • The problem with plate editor v3.4 sample list improperly applying samples with certain sample groups has been determined. This problem has been identified in that a sql statement did not order the samples being queried from the dat
