Is Bacillus subtilis visible in acid-fast stain?
In an acid-fast stain, positive acid-fast bacterium will appear red because they possess a lipid mycolic acid on their membrane and will retain heated carbolfuchsin. Negative acid-fast bacterium will appear blue (stained by methylene blue). Myobacterium and nocardia are negative acid-fast bacterium. All the rest of bacterium (i.e. bacillus) are acid-fast positve.