Is a specific microplate recommended for the BCA Protein Assay?
Although the brand of plate should not affect the BCA Protein Assay, assay results may be affected by well configuration. The microplate protocol is based on the use of clear, flat-bottomed plates. Using round-bottomed plates may affect the A(562) readings because the plate reader may not be able to align the plate within the optical path with reproducibility, which will cause variation in the absorbance from well to well. To achieve optimum results, Pierce recommends the use of new plates and glassware that has been thoroughly cleaned and rinsed before use.
