Important Notice: Our web hosting provider recently started charging us for additional visits, which was unexpected. In response, we're seeking donations. Depending on the situation, we may explore different monetization options for our Community and Expert Contributors. It's crucial to provide more returns for their expertise and offer more Expert Validated Answers or AI Validated Answers. Learn more about our hosting issue here.

Is a RBS present in the vectors pMM152x, pHIS152x, pSTREP152x?

April 26, 2017PRESENT RBS vectors

0

Posted

Is a RBS present in the vectors pMM152x, pHIS152x, pSTREP152x?

1 Answer

0

Posted

A.: All vectors (pMM152x, pHIS152x, pSTREP152x) contain an RBS upstream of the BsrG I site. The only differences between these vectors is the presence of the coding sequence of the signalpeptide in pMM1525, pHIS1525 and pSTREP1525. That means it is possible to clone the gene of interest in the MCS in parallel (in pHIS1525 downstream of the coding sequence of the signal peptide). Please clone into the right reading frame as marked by the Start codon for xylA.